蜂巢矩陣葉綠素熒光成像系統(tǒng)

HEXAGON-IMAGING-PAM

葉綠素熒光成像系統(tǒng)的“六邊形戰(zhàn)士"

精度高，面積大，功能全，應用廣，文獻多，數(shù)據(jù)可視化！

HEXAGON-IMAGING-PAM是德國WALZ公司最新推出的大型蜂巢矩陣葉綠素熒光成像系統(tǒng)。它憑借高精度的脈沖振幅調(diào)制（PAM）技術，可以對20×24cm的區(qū)域進行成像。分辨率高達1.2 MP（1000 x 1200 px, 2x2 binning技術，實際是2000×2400），像素尺寸3.45 x 3.45 µm。

超高分辨率的基礎是成像區(qū)域光場的均勻性，在設計過程中，光源陣列中LED的位置是經(jīng)過精心布局的，以保證測量區(qū)域內(nèi)無陰影，所有成像區(qū)域內(nèi)的樣品均勻照光，樣品間的差異可以盡收眼底。大功率LED面板的冷卻效果非常好，可以最大限度的延長LED的使用壽命。

增加遠紅光（FR）LED 面板，可用于測量所研究樣品的Fo'值。

HEXAGON-IMAGING-PAM采用蜂巢矩陣式LED面板拼接技術，單個六邊形蜂巢矩陣單元之間LED的不平橫可以獨立補償，初衷是為實現(xiàn)樣品區(qū)域的理想照明提供更優(yōu)選擇。

盡管成像區(qū)域很大，但是它依然足夠靈活，可以測量各種類型的樣品，如盆栽植物，穴盤中培養(yǎng)的植物，培養(yǎng)皿上的植物或多孔板中的藻類懸浮液。

滑動門設計，集成安全關閉功能，開門狀態(tài)下，飽和脈沖的強度會被抑制以保護操作人員的眼睛。

主要功能

l 原位測量：活體植物葉綠素熒光成像，直觀顯示樣品光合作用光能利用差異，可導出彩色圖像。

l 成像功能：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護能力，測定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。

l 程序測量功能：可自動程序測量熒光誘導曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數(shù)的變化趨勢；可以預編程進行自定義實驗流程，如模擬波動光。

l AOI功能：可在測量前或測量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣訉x擇的AOI的數(shù)據(jù)進行變化趨勢分析，并在報告文件中顯示相關AOI的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導出到EXCEL文件中。

l 成像異質(zhì)性分析功能：對任意參數(shù)任意時間的成像，可在圖像上任意選取兩點，軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析，并可導出到EXCEL文件中。

l 成像數(shù)據(jù)范圍分析功能：對任意參數(shù)任意時間的成像，可分析任意兩個熒光數(shù)值之間有多少個像素點，多少面積（cm2）。

l 突變株篩選功能：可跟據(jù)成像結果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

l 微藻毒理研究功能：可同時測量4塊96孔板，即384個微藻樣品（對照和處理組）的光合活性，軟件自動給出處理組樣品相對于對照組的光合抑制百分比。

應用領域

l 光合作用研究：可以在*相同的條件下同時對大量樣品進行成像

l 植物病理學：病斑部位（包括肉眼不可見時）成像以及病斑擴散的時空動力學

l 植物脅迫生理學：肉眼不可見生物/非生物脅迫損傷的早期檢測

l 遺傳育種：出苗后大規(guī)模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株

l 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

l 微藻毒理學：不同毒物濃度多個重復的樣品一次測完，軟件自動計算抑制比率

l 其它多種擴展研究

成像參數(shù)

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.等

產(chǎn)地：德國WALZ

參考文獻

數(shù)據(jù)來源：光合作用文獻Endnote數(shù)據(jù)庫，原始數(shù)據(jù)來源：Google Scholar。

注：HEXAGON-IMAGING-PAM為最新產(chǎn)品，暫無文獻發(fā)表，最新研究成果可參考M-IMAGING-PAM發(fā)表文章。

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014年2月，上海澤泉科技股份有限公司在上海浦東孫橋現(xiàn)代農(nóng)業(yè)園區(qū)投資成立了上海乾菲諾農(nóng)業(yè)科技有限公司，建設了AgriPhenoTM “高通量植物基因型-表型-育種服務平臺"，為植物科研和育種單位提供全面的樣品收集和栽培，實驗設計和項目合作，以及表型數(shù)據(jù)與生物信息學分析綜合服務。平臺成功主持了上海張江國家自主創(chuàng)新示范區(qū)專項發(fā)展資金重點項目“澤泉科技高通量植物基因型-表型-育種服務平臺"。作為主持單位或合作單位參與了上海市農(nóng)委和科委的30多項政府科研服務項目以及商業(yè)服務項目，如科技興農(nóng)種業(yè)發(fā)展項目“農(nóng)作物分子育種的技術創(chuàng)新研究"和“青菜高通量表型圖譜標準的建立及主要性狀分析"、科技興農(nóng)重點攻關項目“基于圖像分析及三維建模技術的黃瓜長勢快速評價方法研究"、 “蘭科觀賞花卉分子育種技術研究與產(chǎn)業(yè)化應用"等。為了緊追世界科技發(fā)展水平，開啟院企合作建立研究型平臺的創(chuàng)新嘗試，上海澤泉科技股份有限公司與上海市農(nóng)業(yè)科學院，結合雙方各自的優(yōu)勢，于2021年5月在上海農(nóng)業(yè)科學院莊行試驗站聯(lián)合成立“上海市農(nóng)業(yè)科學院莊行綜合試驗站澤泉科技植物表型技術研究平臺"，AgriPhenoTM平臺從上海浦東孫橋現(xiàn)代農(nóng)業(yè)園區(qū)整體遷出，并入新建的植物表型技術研究平臺。目前平臺除擁有無人機表型平臺、溫室型和實驗室型高通量表型分析系統(tǒng)外，還擁有現(xiàn)代化溫室、生物學實驗室、植物生理生態(tài)測量設備、農(nóng)業(yè)氣象測量系統(tǒng)和專業(yè)的數(shù)據(jù)庫平臺，已經(jīng)具備了對植物、動物基因測序與植物表型研究的各類條件?？梢猿袚咄緿NA提取、基因測序服務、分子輔助育種、植物生理生態(tài)研究等科研實驗任務。同時可以為植物功能基因組、農(nóng)業(yè)育種家提供高通量植物基因型測試、高通量植物表型測試和植物基因型-表型生物信息學數(shù)據(jù)分析等開放式服務。